Premium
Site Distribution in Resin Beads as Determined by Confocal Raman Spectroscopy
Author(s) -
Kress Jürgen,
Rose Abigail,
Frey Jeremy G.,
Brocklesby William S.,
Ladlow Mark,
Mellor Geoff W.,
Bradley Mark
Publication year - 2001
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/1521-3765(20010917)7:18<3880::aid-chem3880>3.0.co;2-#
Subject(s) - confocal , raman spectroscopy , spectroscopy , materials science , distribution (mathematics) , analytical chemistry (journal) , chemistry , optics , chromatography , physics , mathematics , astronomy , mathematical analysis
Abstract Scanning confocal Raman spectroscopy was used to study the distribution of reactive sites within a resin bead used for solid‐phase synthesis. The distribution of NH 2 groups in aminomethylated polystyrene resin (APS) was determined by doping with varying amounts of 4‐cyanobenzoic acid. The extent of loading was determined by both elemental analysis and ninhydrin assays. The spatial distribution of the coupled 4‐cyanobenzamide within the bead was determined to an in‐plane resolution of 1 μm and depth resolution of about 4 μm, using the strong Raman CN stretching vibrational transition at 2230 cm −1 . Dry and swollen beads were studied and the distribution was found to be essentially uniform throughout the bead in all cases.