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Involvement of Fas receptor and not tumor necrosis factor‐α receptor in ultraviolet‐induced activation of acid sphingomyelinase
Author(s) -
Chatterjee Madhumita,
Wu Shiyong
Publication year - 2001
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/1098-2744(200101)30:1<47::aid-mc1012>3.0.co;2-3
Subject(s) - acid sphingomyelinase , fadd , tumor necrosis factor receptor 1 , death domain , biology , fas receptor , ceramide , receptor , tradd , traf2 , apoptosis , tumor necrosis factor alpha , transfection , fas ligand , signal transduction , microbiology and biotechnology , programmed cell death , caspase , biochemistry , immunology , gene , tumor necrosis factor receptor
Abstract Fas receptor and tumor necrosis factor receptor‐1 (TNFR1) mediate the activation of acid sphingomyelinase (ASMase), which catalyzes the hydrolysis of sphingomyelin to ceramide. Ceramide acts as a second messenger in mediating cell growth, differentiation, stress response, and apoptosis. Ultraviolet (UV) irradiation induces Fas receptor and TNFR1 aggregation. However, the roles of Fas receptor and TNFR1 in mediating UV‐induced ASMase activation have not been explored. In this report, we demonstrate that Fas receptor, not TNFR1, mediated UV‐induced activation of ASMase. Our data indicate that ASMase activity was not induced with UV irradiation but by TNFα in MCF‐7 cells that expressed low levels of Fas receptor. In contrast, ASMase was activated by UV irradiation or TNFα treatment in Fas stably transfected MCF‐7 cells. Immunofluorescence staining of TNFR1 on MCF‐7 cells showed that TNFR1 was aggregated after treatment with UV irradiation or TNFα. However, UV‐induced aggregation of TNFR1 did not lead to induction of ASMase activity. These results suggest that Fas receptor aggregation is solely responsible for UV‐induced activation of ASMase. Further, with the use of BJAB and dominant‐negative Fas‐associated death domain–containing protein (FADD) stably transfected BJAB cells, we demonstrated that dominant‐negative FADD partly inhibited UV‐induced ASMase activation. Our results suggest that FADD is involved in UV‐induced and Fas‐mediated signaling pathways for activation of ASMase. Mol. Carcinog. 30:47–55, 2001. © 2001 Wiley‐Liss, Inc.