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Localized activation of m‐calpain in migrating human umbilical vein endothelial cells stimulated by shear stress
Author(s) -
Ariyoshi Hideo,
Yoshikawa Norihide,
Aono Yasuhisa,
Tsuji Yoshihisa,
Ueda Atsushi,
Tokunaga Masaru,
Sakon Masato,
Monden Morito
Publication year - 2001
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/1097-4644(20010401)81:1<184::aid-jcb1034>3.0.co;2-g
Subject(s) - umbilical vein , calpain , shear stress , microbiology and biotechnology , shear (geology) , materials science , chemistry , biology , composite material , biochemistry , enzyme , in vitro
Abstract Using a parallel‐plate flow‐chamber and confocal laser scanning microscopy (CLSM), we studied the mode of cytoskeletal reorganization in migrating HUVECs stimulated by shear stress. Activation of m‐calpain associated with a change in the spatial distribution of cytoplasmic ionized Ca 2+ concentration ([Ca 2+ ] i ) was studied. Shear stress (10 dyne/cm 2 ) caused migration and decrease in the F‐actin content of HUVECs. Migrating individual HUVECs showed the lamellipodium formed in the direction of cell migration, in which [Ca 2+ ] i elevated to 148 ± 12 nM in a localized fashion. We found the appearance of activated m‐calpain in the local area of the migrating HUVECs, which was associated with a decrease in the amounts of pp125FAK and ezrin. The localized rise in [Ca 2+ ] i might be closely related to morphological change to regulate the direction of cell migration induced by shear stress through localized activation of m‐calpain. J. Cell. Biochem. 81:184–192, 2001. © 2001 Wiley‐Liss, Inc.