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Establishment of a new Hodgkin's cell line (HD‐70) of B‐cell origin
Author(s) -
Kanzaki Tetsuya,
Kubonishi Ichiro,
Eguchi Taisuke,
Yano Shoki,
Sonobe Hiroshi,
Ohyashiki Junko H.,
Ohyashiki Kazuma,
Toyama Keisuke,
Ohtsuki Yuji,
Miyoshi Isao
Publication year - 1992
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19920215)69:4<1034::aid-cncr2820690434>3.0.co;2-e
Subject(s) - cd15 , pathology , cd30 , cell culture , microbiology and biotechnology , immunoglobulin light chain , cytoplasm , b cell , biology , antibody , medicine , lymphoma , immunology , genetics , stem cell , cd34
Abstract A new Hodgkin's cell line, designated HD‐70, was established from the peripheral blood of a 69‐year‐old man with Hodgkin's disease of nodular sclerosing type. The cell line grows in a single cell suspension and has a doubling time of 28 hours. The cells have a round or irregular nucleus or multiple nuclei in relatively abundant cytoplasm that is positive for acid phosphatase, alpha‐naphthyl butyrate esterase, and periodic acid‐Schiff stains. HD‐70 cells are positive for CD30 (Ki‐1 JBer‐H2), CD15 (Leu‐Ml), and CD71 (OKT9) antigens and contain cytoplasmic immunoglobulin (Ig) (A, kappa). Southern blot analysis showed that the cells have Ig heavy and kappa light chain gene rearrangement and lack T‐cell receptor gene rearrangement. Chromosome analysis disclosed that the cells have a human karyotype with complicated abnormalities, including a 14q+. Heterotrans‐plantation of the HD‐70 cell line into newborn hamsters treated with antilymphocyte serum produced massive tumors with remarkable fibrosis and collagen band formation. These tumors displayed histologic features similar to those of the nodular sclerosing type tumor of the patient. Such fibrosis production and collagen band formation in heterotransplanted tumors suggest that a certain cytokine that induces fibrosis might be produced by HD‐70 cells. This cell line may be useful for understanding the biology and pathogenesis of Hodgkin's disease. Cancer 1992; 69:1034–1041.