Premium
Role of adenosine kinase in Saccharomyces cerevisiae : identification of the ADO1 gene and study of the mutant phenotypes
Author(s) -
Lecoq K.,
Belloc I.,
Desgranges C.,
DaignanFornier B.
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/1097-0061(20010315)18:4<335::aid-yea674>3.0.co;2-x
Subject(s) - adenosine kinase , biology , adenosine , saccharomyces cerevisiae , biochemistry , gene , mutant , kinase , open reading frame , yeast , cordycepin , protein kinase a , adenosine deaminase , peptide sequence
Abstract Sequencing of the Saccharomyces cerevisiae genome revealed an open reading frame ( YJR105w ) encoding a putative protein highly similar to adenosine kinases from other species. Disruption of this gene (renamed ADO1 ) affected utilization of S‐adenosyl methionine (AdoMet) as a purine source and resulted in a severe reduction of adenosine kinase activity in crude extracts. Furthermore, knock‐out of ADO1 led to adenosine excretion in the medium and resistance to the toxic adenosine analogue cordycepin. From these data we conclude that ADO1 encodes yeast adenosine kinase. We also show that ADO1 does not play a major role in adenine utilization in yeast and we propose that the physiological role of adenosine kinase in S. cerevisiae could primarily be to recycle adenosine produced by the methyl cycle. Copyright © 2001 John Wiley & Sons, Ltd.