Premium
A novel normalized retention factor in micellar electrokinetic chromatography
Author(s) -
Idei Miklós,
Győrffy Erika,
Kiss Éva,
Őrfi László,
Seprődi János,
Tamás Boróka,
Hollósy Ferenc,
Mészáros György,
Kéri György
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990601)20:7<1561::aid-elps1561>3.0.co;2-0
Subject(s) - alkylbenzenes , micellar electrokinetic chromatography , electrokinetic phenomena , chemistry , analyte , chromatography , function (biology) , analytical chemistry (journal) , capillary electrophoresis , organic chemistry , benzene , evolutionary biology , biology
Abstract Characteristic properties of the expression k ″ = ( t m — t o )/( t mc — t o ) and its applicability in micellar electrokinetic capillary chromatography (MEKC) were compared to the previous expression, k ′ = ( t m — t o )/ t o (1‐ t m / t mc ), introduced by Terabe. It was proved with theoretical calculations (curve shape analysis) that the properties of function k ″ are in full accordance with the properties of the MEKC system and k ″ could be applied advantageously to characterize hydrophobicity of the analytes. This conclusion is now supported by experimental data obtained with homolog series of alkylbenzenes and alkylphenones as well as with hydrophobic protected peptides. Migration times, k ′, k ″ values, and software‐calculated hydrophobicity data are summarized and analyzed in the present study. Since k ″ is a normalized parameter, good relationships between the migration time, the software‐calculated hydrophobicity, and the k ″ values were obtained. Differences in hydrophobicity of the analytes could be estimated in a more realistic way with the aid of function k ″ than by using function k ′. Hydrophobicity data estimated on the basis of the k ″ values proved to be in good accordance with the expectations based on the migration times and on the chemical structures of the compounds investigated.