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NK cells differentiated from bone marrow, cord blood and peripheral blood stem cells exhibit similar phenotype and functions
Author(s) -
Carayol Géraldine,
Robin Catherine,
Bourhis JeanHenri,
BennaceurGriscelli Annelise,
Chouaib Salem,
Coulombel Laure,
Caignard Anne
Publication year - 1998
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/(sici)1521-4141(199806)28:06<1991::aid-immu1991>3.0.co;2-7
Subject(s) - biology , interleukin 12 , interleukin 21 , lymphokine activated killer cell , cytotoxic t cell , janus kinase 3 , bone marrow , cd34 , stem cell factor , stromal cell , cord blood , stem cell , microbiology and biotechnology , immunology , progenitor cell , cancer research , in vitro , t cell , immune system , biochemistry
Abstract In the present study, we investigated the differentiation of human NK cells from bone marrow, cord blood and mobilized peripheral blood purified CD34 + stem cells using a potent culture system. Elutriated CD34 + stem cells were grown for several weeks in medium supplemented with stem cell factor (SCF) and IL‐15 in the presence or absence of a murine stromal cell line (MS‐5). Our data indicate that IL‐15 induced the proliferation and maturation of highly positive CD56 + NK cells in both types of culture, although murine stromal cells slightly increased the proliferation of NK cells. NK cells differentiated in the presence of MS‐5 were mostly CD56 + CD7 − and a small subset expressed CD16. These in vitro differentiated CD56 + NK cells displayed cytolytic activity against the HLA class I − target K562. The CD56 + CD16 + subset also lysed NK‐resistant Daudi cells. Neither of these NK subsets were shown to express Fas ligand. Total CD56 + cells expressed high amounts of transforming growth factor‐β and granulocyte‐macrophage colony‐stimulating factor, but no IFN‐γ. Investigation of NK receptor expression showed that most CD56 + cells expressed membrane CD94 and NKG2‐A mRNA. PCR analysis revealed that p58 was also expressed in these cells. The role of CD94 in NK cell‐mediated cytotoxicity was assessed on human HLA‐B7‐transfected murine L cells. While a low cytotoxic activity towards HLA‐B7 cells was observed, the HLA‐DR4 control cells were killed with high efficiency. These studies demonstrate that cytolytic and cytokine‐producing NK cells may be derived from adult and fetal precursors by IL‐15 and that these cells express a CD94 receptor which may influence their lytic potential.