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Transcription level of operon ftsYEX and activity of promoter P1 of rpoH during the cell cycle in Escherichia coli
Author(s) -
GómezEichelmann M. Carmen,
Helmstetter Charles E.
Publication year - 1999
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/(sici)1521-4028(199909)39:4<237::aid-jobm237>3.0.co;2-4
Subject(s) - transcription (linguistics) , operon , biology , promoter , microbiology and biotechnology , gene , cell division , terminator (solar) , escherichia coli , genetics , cell , gene expression , ionosphere , philosophy , linguistics , physics , astronomy
Abstract In Escherichia coli , the genes of the main heat‐shock proteins are under the control of the product of gene rpoH , protein σ 32 . The distal promoter P1 of rpoH is located within the terminator of the division operon ftsYEX which could imply some coupling between ftsYEX transcription termination, P1 transcription and cell division. To study the possibility of this coupling, the level of transcription of ftsYEX and the activity of promoter P1 of rpoH were examined in synchronous cultures. Results indicate that transcription of ftsYEX and of rpoH from P1 is continuous, suggesting that ftsYEX transcription termination and P1 activity are not coupled to the cell cycle.