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A New Co II Complex as a Bulge‐Specific Probe for DNA
Author(s) -
Cheng ChienChung,
Kuo YenNing,
Chuang KuoShen,
Luo ChiFong,
Wang Wen Jwu
Publication year - 1999
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19990503)38:9<1255::aid-anie1255>3.0.co;2-t
Subject(s) - dna , radical , cleavage (geology) , chemistry , hydrogen peroxide , reactivity (psychology) , octahedron , stereochemistry , ligand (biochemistry) , substrate (aquarium) , crystallography , biochemistry , biology , crystal structure , receptor , medicine , paleontology , ecology , alternative medicine , pathology , fracture (geology)
Abstract Bulge cleavage of two or three bases occurs when a DNA substrate is specifically cleaved oxidatively by [Co II (tfa) 2 (happ)] (see picture). Hydrogen peroxide is necessary for the activation of this octahedral complex, which suggests that hydroxyl radicals are the reactive species. The complex has no significant reactivity towards the corresponding sequence in a single‐stranded DNA region, and it exhibits only a low affinity towards double‐stranded DNA. happ=macrocyclic ligand based on 1,10‐phenanthroline, tfa=trifluoroacetate.