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Induction of stress proteins by electromagnetic fields in cultured HL‐60 cells
Author(s) -
Pipkin J.L.,
Hinson W.G.,
Young J.F.,
Rowland K.L.,
Shaddock J.G.,
Tolleson W.H.,
Duffy P.H.,
Casciano D.A.
Publication year - 1999
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/(sici)1521-186x(199909)20:6<347::aid-bem3>3.0.co;2-i
Subject(s) - stress (linguistics) , microbiology and biotechnology , bioelectromagnetics , electromagnetic field , physics , biology , immunology , philosophy , linguistics , quantum mechanics
Abstract HL‐60 cells in culture were exposed for 2 h to a sinusoidal 0.1 or 1 mT (1 or 10 Gauss) magnetic field at 60 Hz and pulse labeled after exposure with radioactive isotopes by incubation by using either [ 35 S]methionine, [ 3 H]leucine, or [ 33 P]phosphate. The radioactive labels were incorporated into cellular proteins through synthesis or phosphorylation. Proteins were extracted from electrostatically sorted nuclei, and the heat shock/stress proteins (sp) were analyzed for synthesis and phosphorylation by two‐dimensional polyacrylamide gel electrophoresis. In the control cultures (no exposure to the magnetic field), sp 72c (cognate form) was faintly observed. A 0.1 mT exposure did not show sp metabolism to be different from that of the controls; however, after a 1 mT exposure of the HL‐60 cells, sp 70i (inducible form) was synthesized ([ 35 S]methionine incorporation). Sp 90 was not synthesized at either field level, but was phosphorylated ([ 33 P]phosphate incorporation) in the 1 mT exposure. Sp 27 (isoforms a and b) was induced after a 1 mT exposure as reflected by labeling with [ 3 H]leucine. These sps were not detected after a 0.1 mT exposure. After a 1 mT exposure and labeling with [ 33 P], sp 27 isoforms b and c were phosphorylated whereas isoform ‘a‘ was not observed. Sps 70i, 72c, and 90 were identified by commercial sp antibodies. Likewise, polypeptides a, b, and c were verified as sp 27 isoforms by Western blotting. Statistical evaluation of sp areas and densities, determined from fluorographs by Western‐blot analysis, revealed a significant increase in sps 90 and 27a after a 1 mT magnetic field exposure. The 1 mT magnetic field interacts at the cellular level to induce a variety of sp species. Bioelectromagnetics 20:347–357, 1999. Published 1999 Wiley‐Liss, Inc.