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Isothermal titration calorimetry and differential scanning calorimetry as complementary tools to investigate the energetics of biomolecular recognition
Author(s) -
Jelesarov Ilian,
Bosshard Hans Rudolf
Publication year - 1999
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/(sici)1099-1352(199901/02)12:1<3::aid-jmr441>3.0.co;2-6
Subject(s) - isothermal titration calorimetry , differential scanning calorimetry , energetics , chemistry , calorimetry , molecule , biomolecule , isothermal process , isothermal microcalorimetry , titration , energetic material , protonation , thermodynamics , enthalpy , organic chemistry , biochemistry , ion , physics , explosive material
Abstract The principles of isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) are reviewed together with the basic thermodynamic formalism on which the two techniques are based. Although ITC is particularly suitable to follow the energetics of an association reaction between biomolecules, the combination of ITC and DSC provides a more comprehensive description of the thermodynamics of an associating system. The reason is that the parameters Δ G, Δ H, Δ S, and Δ C p obtained from ITC are global properties of the system under study. They may be composed to varying degrees of contributions from the binding reaction proper, from conformational changes of the component molecules during association, and from changes in molecule/solvent interactions and in the state of protonation. Copyright © 1999 John Wiley & Sons, Ltd.