Effect of Mobile Phase Composition on Selectivity in Preparative Hydrophobic Interaction Chromatographic Purification of Glucagon | Zendy

Angelova M. | Zendy; Dimov N. | Zendy

Premium

Effect of Mobile Phase Composition on Selectivity in Preparative Hydrophobic Interaction Chromatographic Purification of Glucagon

Author(s) -

Angelova M.,

Dimov N.

Publication year - 1996

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/(sici)1099-0801(199609)10:5<251::aid-bmc601>3.0.co;2-o

Subject(s) - chemistry , amberlite , chromatography , selectivity , glucagon , composition (language) , impurity , hydrophobic effect , chromatographic separation , hydrophilic interaction chromatography , phase (matter) , high performance liquid chromatography , catalysis , organic chemistry , biochemistry , adsorption , hormone , linguistics , philosophy

A weakly hydrophobic acrylester copolymer, Amberlite XAD‐7, has been used in the purification process of glucagon production for elimination of protein impurities in the crude glucagon fractions, obtained from the industrial waste from insulin production. Varying the mobile phases composition, it was shown that suitable selectivity for the separation of proteins under non‐denaturing conditions can be achieved. The purified glucagon, obtained at the proposed chromatographic conditions has a biological activity of above 1 IU/mg.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research