Open AccessSensitive enzyme‐linked immunosorbent assay for adult T‐cell leukemia‐derived factor and normal value measurement
Author(s) -
Kogaki Hiroyuki,
Fujiwara Yuko,
Yoshiki Akemi,
Kitajima Sachiko,
Tanimoto Tetsuji,
Mitsui Akira,
Shimamura Toshiro,
Hamuro Junji,
Ashihara Yoshihiro
Publication year - 1996
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/(sici)1098-2825(1996)10:5<257::aid-jcla5>3.0.co;2-5
Subject(s) - monoclonal antibody , microbiology and biotechnology , recombinant dna , enzyme , leukemia , thioredoxin , chemistry , monoclonal , antibody , biology , immunology , biochemistry , gene
Abstract Four different monoclonal antibodies against recombinant adult T‐cell leukemia‐derived factor (ADF), identical to thioredoxin, were established and used for the determination of ADF concentration in serum. Using two of the monoclonal antibodies, we developed a two‐step enzyme‐linked immunosorbent assay (ELISA) for ADF. This ELISA showed a highly specific reactivity on ADF with no cross‐reactivity to several proteins with homologue sequence on the active center. The detection limit of the assay was 2.0 ng/ml (mean ± 2 SD). The intra‐ and interassay coefficients of variation (CV) were 0.81–3.74% (n = 8) and 4.78–6.97% (n = 7), respectively. The normal value of ADF mean concentration from 145 healthy donors was 40.8 ng/ml. © 1996 Wiley‐Liss, Inc.