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Kinetic study on the esterification of geraniol and acetic acid in organic solvents using surfactant‐coated lipase
Author(s) -
Huang ShihYow,
Chang HsiaoLi
Publication year - 1999
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199902)74:2<183::aid-jctb3>3.0.co;2-h
Subject(s) - acetic acid , chemistry , lipase , geraniol , pulmonary surfactant , organic chemistry , flavor , candida antarctica , solvent , triacylglycerol lipase , solubility , catalysis , chromatography , enzyme , biochemistry , essential oil
Surfactant‐coated lipase (SCL) was prepared bycoating the surface of Candida cylindracea lipase with thenon‐ionic surfactant, Span 85. SCL catalyzed theesterification of geraniol and acetic acid. The ester product,geranyl acetate, is an important component of flavor and fragrancecompounds. The SCL prepared in this work had low solubility inorganic solvent, unlike those prepared by other investigators. Akinetic study of the mechanism of the lipolytic esterification andthe reaction rate for facilitating the scale‐up of thereactors indicated a ping‐pong bi bi model with competitivesubstrate inhibition by acetic acid and dead‐end inhibition byacetic acid. Experimental data agreed with the proposed model.Kinetic parameters, K i , K m (AA) , K m (GOH) and V max , were determined which visualizedthe reaction mechanism of the SCL esterification. © 1999 Society of Chemical Industry
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