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Induction of the ATP‐sensitive potassium (uK ATP ‐1) channel by endotoxemia
Author(s) -
Czaika G.,
Gingras Y.,
Zhu E.,
Comtois A.S.
Publication year - 2000
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/(sici)1097-4598(200006)23:6<967::aid-mus19>3.0.co;2-p
Subject(s) - potassium channel , blot , sepsis , atp sensitive potassium channel , potassium , messenger rna , microbiology and biotechnology , saline , chemistry , reverse transcriptase , gene expression , protein expression , medicine , endocrinology , andrology , biology , polymerase chain reaction , biochemistry , gene , glibenclamide , organic chemistry , diabetes mellitus
The effect of sepsis on the ubiquitously expressed ATP‐sensitive potassium (uK ATP ‐1) channel expression was measured in Sprague‐Dawley rat diaphragms. Rats were treated with either 0.5 ml saline or 20 mg/Kg E. coli lipopolysaccharides and sacrificed at 3, 6, 12, 24, or 48 h later. Reverse transcriptase‐polymerase chain reaction (RT‐PCR) analysis showed that channel mRNA expression was increased at 3 h and continued to rise up to 48 h. Western blotting analysis showed a ∼9‐fold increase in channel protein expression 24 h after sepsis. Our results demonstrate that sepsis upregulates the uK ATP ‐1 channel. © 2000 John Wiley & Sons, Inc. Muscle Nerve 23: 967–969, 2000
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