Premium
Early cortical precursors do not undergo LIF‐mediated astrocytic differentiation
Author(s) -
Molné Montse,
Studer Lorenz,
Tabar Viviane,
Ting YuanTsang,
Eiden Maribeth V.,
McKay Ronald D.G.
Publication year - 2000
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(20000201)59:3<301::aid-jnr3>3.0.co;2-h
Subject(s) - ciliary neurotrophic factor , leukemia inhibitory factor , nestin , biology , cellular differentiation , microbiology and biotechnology , precursor cell , population , gliogenesis , neuroscience , stem cell , neural stem cell , neural crest , receptor , cell , immunology , neurotrophic factors , cytokine , embryo , interleukin 6 , medicine , biochemistry , environmental health , gene
Abstract Multipotential stem cells have been isolated from the developing and adult CNS. Similar identified factors control the differentiation of these cells. A striking example is the instructive action of CNTF/LIF activating the JAK/STAT pathway to induce astrocytic differentiation in both fetal and adult CNS stem cells. Here we show that E12 cortical precursors express functional LIF receptors but do not exhibit this differentiation response to CNTF/LIF either in explant or in dissociated cell culture. The lack of response to LIF‐induced astrocytic differentiation is maintained in cocultures with LIF responsive cells derived from E15 cortex. This suggests cell intrinsic differences between early and late stage precursors in the interpretation of LIF‐mediated signaling; however, the early nestin‐positive precursor population differentiates into both neurons and neural crest derivatives. These data define differences between CNS stem cells from different stages of cortical development. J. Neurosci. Res. 59:301–311, 2000. Published 2000 Wiley‐Liss, Inc.