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Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric detection of bacterial biomarker proteins isolated from contaminated water, lettuce and cotton cloth
Author(s) -
Holland Ricky D.,
Rafii Fatemeh,
Heinze Thomas M.,
Sutherland John B.,
Voorhees Kent J.,
Lay, Jackson O.
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(20000530)14:10<911::aid-rcm965>3.0.co;2-c
Subject(s) - chemistry , shigella flexneri , contamination , aeromonas hydrophila , chromatography , mass spectrometry , matrix (chemical analysis) , escherichia coli , desorption , bacteria , environmental chemistry , microbiology and biotechnology , biochemistry , adsorption , ecology , genetics , organic chemistry , biology , gene
Abstract Matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) mass spectra of bacterial proteins were obtained from water, lettuce and cloth samples contaminated with Shigella flexneri, Escherichia coli , and Aeromonas hydrophila . Spectra were obtained using proteins directly isolated from water (or water used for rinsing samples) without culturing the bacteria. For S. flexneri and E. coli , two marker ions for specific proteins associated with a virulence‐related property (acid resistance) were easily detected. For A. hydrophila , ions from two specifically selected marker proteins, as well as ions from the larger group of proteins isolated from pure cultures, all matched spectra from a contaminated water sample, providing strong evidence that A. hydrophila was the bacterial contaminant. Rinse water from contaminated lettuce and cloth samples showed the same marker ions as the contaminated water samples. Copyright © 2000 John Wiley & Sons, Ltd.