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Characterization of a newly established endometrial stromal sarcoma cell line
Author(s) -
Gunawan Bastian,
Braun Stefan,
Cortés Maria José,
Bergmann Frank,
Karl Christian,
Füzesi László
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980729)77:3<424::aid-ijc19>3.0.co;2-7
Subject(s) - biology , stromal cell , sarcoma , pathology , cell culture , endometrial stromal sarcoma , mesenchymal stem cell , karyotype , cellular differentiation , immunocytochemistry , phenotype , cancer research , microbiology and biotechnology , genetics , chromosome , gene , medicine , endocrinology
Abstract We describe a newly established human sarcoma cell line derived from an endometrial stromal sarcoma (ESS). The cell line has been maintained in long‐term cell culture for more than 2 years. It has been repeatedly analyzed in terms of morphology, immunocytochemical features, ultrastructure and karyotypic characteristics. In contrast to uniform endometrial stromal differentiation in vivo , the tumor cells were shown to display distinct phenotypical heterogeneity in vitro . In addition to the predominant cell type, which retained sarcomatous differentiation, foci of epithelial‐like cells were observed in the cell culture. Immunocytochemical and ultrastructural analysis demonstrated a mainly mesenchymal phenotype with signs of epithelial characteristics, such as expression of cytokeratins, and the presence of desmosomes and kinetocilia, respectively. Cytogenetic analyses in early and late passages revealed unbalanced translocations between chromosomes 3 and 6 and an additional i(19)(q10), as common karyotypic changes in all tumor cells, indicating a monoclonal origin. Our new cell line can be used as an in vitro model to study the mechanisms of heterogenous differentiation patterns in ESS. Int. J. Cancer 77:424–428, 1998. © 1998 Wiley‐Liss, Inc.