Reflection contrast microscopy for high resolution detection of  3 H‐estradiol in ultrathin sections of human stratum corneum | Zendy

Neelissen Jan A. M. | Zendy; Schrijvers Ad H. G. J. | Zendy; Junginger Hans E. | Zendy; Boddé Harry E. | Zendy

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Reflection contrast microscopy for high resolution detection of 3 H‐estradiol in ultrathin sections of human stratum corneum

Author(s) -

Neelissen Jan A. M.,

Schrijvers Ad H. G. J.,

Junginger Hans E.,

Boddé Harry E.

Publication year - 1999

Publication title -

microscopy research and technique

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.536

H-Index - 118

eISSN - 1097-0029

pISSN - 1059-910X

DOI - 10.1002/(sici)1097-0029(19991115)47:4<286::aid-jemt6>3.0.co;2-g

Subject(s) - stratum corneum , osmium tetroxide , microscopy , electron microscope , resolution (logic) , transmission electron microscopy , fixation (population genetics) , materials science , osmium , optical microscope , chemistry , scanning electron microscope , optics , pathology , nanotechnology , medicine , computer science , biochemistry , physics , artificial intelligence , composite material , gene , ruthenium , catalysis

A single autoradiographical method for light and electron microscopy (LM and EM) is presented. Human skin, containing 3 H‐estradiol ( 3 H‐E2) after an in vitro permeation experiment, was processed via a non‐extractive tissue preparation protocol, comprising cryo‐fixation, freeze‐drying, osmium tetroxide vapor fixation, and Spurr resin embedding. Semithin sections were processed for LM autoradiography, while ultrathin sections were processed both for high‐resolution LM and EM autoradiography. The autoradiographs were visualized by bright‐field microscopy (BFM), reflection contrast microscopy (RCM), and transmission electron microscopy to evaluate the potentials of RCM visualization in high‐resolution LM autoradiography. RCM visualization of ultrathin vs. semithin resin sections showed an improved stratum corneum morphology. Histological staining was superfluous. The localization of 3 H‐E2 in human stratum corneum using high‐resolution LM autoradiography and RCM was as accurate as with high‐resolution EM autoradiography. Microsc. Res. Tech. 47:286–290, 1999. © 1999 Wiley‐Liss, Inc.

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