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Label-free combined CARS and SHG microscopy techniques are used as powerfultool to follow the cells behavior in cell-scaffold construct for regenerationof tissues. Imaging of histological section of hDFs seeded in fibrin gelscaffold and imaging of collagen produced by hDFs in a time course experimentat different culture days (0, 7, 21, 42) is performed. A study on the limit ofcollagen detection of the imaging system is reported using sample prepared withdifferent collagen concentrations. The results show that also the small amountof collagen produced by hDFs after few hours of incubation in fibrin gel isdetected. Co-localization of hDFs and collagen is also reported in function ofthe culture days.

Multimodal CARS and SHG microscopy for label-free detection of collagen produced by hDFs in fibrin gel