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Medicinal plants with proven wound healing capacity and antimicrobial contents are analysed based on previous studies. Finally Terminalia chebula extract, Azadirachta indica seed oil, Hemigraphis colorata extracts were selected for the preparation of Ayurvedic formulation. Different concentrations were tried on a trial and error basis and the content ratio was finalised. Formulation was prepared with utmost care under expert supervision. The physical and chemical properties of wound healing cream were investigated, High power thin layer chromatography investigations were done to separate the chemical contents. Inhibition zone diameters were measured to study antimicrobial activity against pathogenic bacteria like Eshcherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus etc.

Ayurvedic preparation from Azadirachta indica, Terminalia chebula, Hemigraphis colorata extracts and Its Antimicrobial investigation