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2gms of liver tissue was collected from Heteropneustus fossilis (fish) and Caprine barbari (goat) for extraction and estimation of DNA, RNA and proteins. Nucleoproteins are soluble in solution of high ionic strength but insoluble in solution of low ionic strength. This property was used for extraction and estimation of DNA by diphenylamine. RNA was extracted by trichloroacetic acid and quantitative estimation was made using oriconol reagent. Total proteins were extracted and estimated by biuret method. DNA extracted was 0.42mg, RNA 3.34mg and proteins 13.4mg from 2gms of liver tissue of Heteropneustes fossilis (fish) and from 2gms of liver tissue of Caprine barbari (goat) DNA estimated was 0.57mg, RNA 2.5mg and proteins were 12.4mg. It is seen that proteins estimated are more than DNA and RNA in given amont of tissue.

Extraction and quantitative estimation of nucleic acids and proteins from liver tissue of Heterpneustus fossilis (fish) and Caprine barbari (goat)