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The present study is concerned with the production of amylase by Bacillus species strain. In this study 12 bacterial strains were isolated and screened for their α-amylase activity. These strains were maintained on nutrient agar medium. Fermentation for the production of amylase was carried out in the enzyme production medium (EPM). All the 12 strains were tested for amylase production. On the basis of maximum amylase activity strain no.1 was selected for further studies. Different starch concentrations, 0.75,1.00,1.25%, pH labels 6.5,7.0,7.5,8.0, aeration (RPM), 100,120,140, temperatures 25 0 C,28 0 C,37 0 C, and 40 0 C and inoculums level 0.5%,1.0%, 1.5% and 2.0% were studied.

Effect of Different Physico-Chemical Parameters on Production ofAmylase by Bacillus Species.