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Decalcification is the commonly employed technique in histopathology laboratories as a part of calcified tissue preparation for the microscopic examination. Chemical agents are most commonly used for routine decalcification procedures, though some agents adversely affect the tissue integrity and staining properties.

journal of clinical and diagnostic research

Qualitative Histological Evaluation of Hard and Soft Tissue Components of Human Permanent Teeth Using Various Decalcifying Agents - A Comparative Study