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Ozet Amac. Cryptosporidium spp. ve Giardia intestinalis insanda ishal nedeni olabilen onemli protozoonlardir. Bu parazitozlarin tanisinda etkensel tani yontemlerinin yaninda antijen tarama testleri de kullanilabilmektedir. Calismamizda ishalli hastalarda konvansiyonel yontemlerle birlikte Direkt Fluoresan Antikor (DFA) yontemi ile G.intestinalis ve Cryptosporidium spp. varliginin saptanmasi ve bu iki protozoonun gastroenterit olgularindaki rolunun arastirilmasi amaclanmistir. Yontem. Sivas il merkezindeki uc hastanenin (Cumhuriyet Universitesi Uygulama ve Arastirma Hastanesi, Sivas Numune ve Sivas Devlet Hastanesi) cesitli servislerine enterit yakinmalariyla basvuran 32’si kadin, 68’i erkek toplam 100 ishalli hastaya ait diski orneklerinde direkt inceleme ve asit-fast boyama yontemi ile yapilan mikroskobik incelemelerde parazitlere ait kist ve/veya ookistler, DFA yontemi ile parazitlere ait antijenler arastirilmistir. Incelenen grupta yer alan hastalar 0-80 yas araliginda bulunmaktadir. Bulgular. Calismada, ishalli 100 diski orneginin 2’sinde direkt mikroskobide G.intestinalis gorulmustur. Asit-fast boyama ile birinde Cryptosporidium spp. gorulurken, DFA ile 4’unde (%4,0) Cryptosporidium spp., 2’sinde (%2,0) G. intestinalis antijenlerine rastlanmistir. Cryptosporidium spp. saptanan hastalarin eriskin yas grubunda yer almasi ilginc bulunmustur. Sonuc. Cryptosporidium spp. ve G. intestinalis’in ikisini de iceren DFA testleri rutin calismalarda ve prevalans arastirmalarinda kullanilabilen kolay, duyarli ve guvenilir bir testtir. Diger taraftan enterit nedenleri arasinda sayilan bu iki protozoon incelenen hasta grubunda toplam %6 oraninda bulunmustur. Anahtar sozcukler: Cryptosporidium spp., giardia intestinalis, DFA, ishal Abstract Aim. Cryptosporidium spp. and Giardia intestinalis are important parasitic protozoan causing diarrhea in developing and developed countries. Diagnosis of the Cryptosporidium oocyst in stool samples by conventional microscopy is labor-intensive and time-consuming. Thus, we aimed the presence of these protozoa in patients with diarrhea and to evaluate the usefulness of direct fluoresan antibody (DFA) test in detecting Cryptosporidium spp and G.intestinalis. from fecal specimens. Methods. For this aim, microscopy and specific antigen detection methods were used to determine Cryptosporidium spp. and G.intestinalis. One hundred stool specimens were examined taken from patients with diarrhea whose ages ranged from 0 to 80 of applied to Hospital of Cumhuriyet University, Numune and Goverment Hospital in Sivas. All samples were tested for Cryptosporidium spp. G.intestinalis antigen by DFA and oocysts via gold Standard modified acid-fast staining for Cryptosporidium spp.and G.intestinalis direct microscopy. Results. One specimen was found to be positive by modified acid-fast staining method and four specimens by DFA method were found to be positive for cryptosporidiosis. On the other hand two specimens were found to be positive both direct microscopy and DFA for giardiosis. Conclusion. The results of DFA test indicate that the simple, rapid, reliable, and standardized test is sensitive and specific for routine diagnosis and may be useful for large-scale epidemiological studies of cryptosporidiosis and giardiosis. Keywords: Cryptosporidium spp., giardia intestinalis, DFA, diarrhea

İshalli hastalarda direkt fluoresan antikor-dfa yöntemi ile giardia ve cryptosporidium spp. araştırılması