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Pyrrolosporin A (1) is a new macrolide antitumor antibiotic possessing an unusual spiro-alpha-acyltetronic acid moiety. The antibiotic was isolated from the fermentation broth of Micromonospora sp. by vacuum liquid chromatography, crystallization and reversed phase HPLC (C18). The structure was determined by a combination of NMR, MS, IR, UV, X-ray analysis and degradation studies.

journal of antibiotics

Pyrrolosporin A, a New Antitumor Antibiotic from Micromonospora sp. C39217-R109-7. II. Isolation, Physico-chemical Properties, Spectroscopic Study and X-ray Analysis.