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In a purification study of novel vasodilator FK409 from the fermentation broth of Streptomyces griseosporeus, we found the existence of FR-900411, a precursor of FK409 in the broth, suggesting that FK409 was derived from FR-900411 via a chemical reaction. We therefore isolated FR-900411 from the broth. The structure of FK409 and FR-900411 were determined to be 1 and 2 respectively, on the basis of spectroscopic and chemical evidence.

journal of antibiotics

FK409, a novel vasodilator isolated from the acid-treated fermentation broth of streptomyces griseosporeus. II. Structure of FK409 and its precursor FR-900411.