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Structure 1 was reported for a novel antifungal agent, L-657,398, isolated from fermentations of Aspergillus ochraceusP However, the relative and absolute stereochemistry of this compound were not reported. We have isolated a compound with the same structure (exclusive of stereochemistry) from fermentations of Preussia sp., by extraction of the mycelial cake with methanol and purification of the antibiotic by repetitive silica gel chromatography eluting with CHCl3-MeOH (98:2) and toluene-MeOH (95:5). Two liters of whole broth (360g of

The relative and absolute stereochemistry of the antifungal agent preussin.