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Sparassis laminosa Fr. is a valuable edible fungus and is protected in many countries, including Ukraine. The study of this fungus in the laboratory and its reproduction under natural conditions are research priorities to preserve this species. We studied the culture growth S. laminosa strain 2211 on nutrient agar media and different plant substrates. Grain-containing substrates were optimal for mycelial growth, the colonization of which occurred from days 10-20 of the experiment. S. laminosa strain 2211 can be grown in the natural environment using the re-situ technique. However, fruit bodies were observed in only one location (near Quercus robur in a sunny area of a temperate deciduous forest, territory of the village Verbovets) at four months after inoculation of the composite substrate containing wheat grain: coniferous sawdust: sunflower seed shells: wheat straw at a ratio of 67%:17%:8%:8%. The obtained fruit bodies were typical of the species morphology, but they had a considerably smaller size. In addition, only substrates that were completely colonized with mycelium were suitable for the application of S. laminosa in nature. The use of partially colonized substrates was accompanied by contamination and growth of Schizophyllum commune fruit bodies. Monitoring of the periodicity of the fruiting within the fixed locality and the effectiveness of all 15 the substrates used in experiment for the fruiting of S. laminosae strain 2211, will be conducted in future studies.

current research in environmental and applied mycology (journal of fungal biology)

Biological features of Sparassis laminosa Fr. (Sparassidaceae, Polyporales) and the main aspects of its reproduction in the territory of Hutsulshchyna National Natural Park, Ukraine