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The isolates TR-B 98 (2) and TR-B 98 (3) from the phosphate mines of Morocco and the isolate Trichoderma asperellum were tested for their ability to survive in the soil and stimulate the growth of wheat plants (soft wheat and durum wheat) and barley on a Mamora (SM) sand-based growing medium modified with increasing concentrations of natural phosphate. Trichoderma isolates have shown positive effects on the germination of wheat seeds (soft and hard) and barley, on the growth and yield of plants growing on substrates containing 0%, 25% and 50% phosphate natural. However, their effect was less on plants growing on substrates containing 75 and 100% natural phosphate. They have also shown their ability to survive in different growing media with differing colonization percentages of straw fragments and roots which increases with time. However, the number of units forming Trichoderma colonies per gram of soil has decreased over time.

Effects of Trichoderma on growth and yield of wheat and barley and its survival ability on roots and amended rock phosphate growing substrates