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The objective of this study was to examine influence of glycerol and guar-xanthan in different concentrations on bi-layer film based on pumpkin oil cake (PuOC) and corn zein, and to find optimal concentrations of these additives which result in film with optimal mechanical and barrier characteristics. Different concentrations of glycerol (30%, 40%, and 50%) as plasticizer and guar-xanthan (0.1%, 0.3%, and 0.5%) as a stabilizer were added in PuOC layer, while zein layer stayed unchanged in all film compositions. Obtained results showed that the optimal mechanical properties had film with the lowest concentration of glycerol (30%) and the highest concentration of guar-xanthan (0.5%). Films showed poor barrier for water vapour, typical for biopolymer-based films. However, all tested films were very good barrier to O2 (transmission rate lower than 50 ml/m2/day at 1 bar). On the other hand, with increase of concentration of added substances, transmission rate of CO2 increased

Influence of different concentrations of glycerol and guar xanthan on properties of pumpkin oil cake-zein bi-layer film