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Eryngium amethystinum L. belonging to the Apiaceae family, is a perennial plant distributed in Southeast Europe. Ev en though this plant is used in traditional medicine, its phytochemical characteriz ation is still incomplete. In this study composition of bioactive constituents of the m thanol extract are reported for the first time. By means of the UPLC-LTQ-Orbitr ap-MS method, altogether sixty-three constituents were characterized: eight hydroxybenzoic acid derivatives (7-13, 32), fifteen cinnamic acid derivatives (14, 17-19, 214-26, 28, 30, 39-42 and 44), four flavonoid aglycones (45, 51, 52, 54), twenty-four flavonoid derivatives (23, 27, 29, 31, 33-38, 43, 46-50, 53, 5-59, 61 and 62), three coumarin derivatives (15, 16 and 22) and nine other compounds (1-6, 20, 60 and 63).

The investigation of bioactive secondary metabolites of the methanol extract of eryngium amethystinum