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Synergistic activity between water, acetone, ethano l and diethyl ether extract of Agrimonia eupatoria L. and commonly used antibiotic (ampicillin) were evaluated. Escherichia coli, Proteus mirabilis, Klebsiella pneumoniae and Pseudomonas aeruginosa were used. Interaction between plant extracts and antibiotic were tested by checkerboard method and expressed as fractional inh ibitory concentration (FIC) index showed indifferent, additive and synergistic effect s. Synergism was observed against E. coli for every combination of agents. FICI values were r anged from 0.03 to 0.29. Inhibitory concentration (IC50) was evaluated for every combination of tested ext racts and antibiotic and the best combinations for every test ed bacteria were combination of diethyl ether extract + ampicillin and combination of aceto n extract + ampicillin.

In vitro interaction between Agrimonia eupatoria L.: Extracts and antibiotic