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Neuromuscular electrical stimulation (NMES) is the action of therapeutic electrical stimulation on muscle tissue in order to contract the muscle and consequently improve the muscle status. Objective: To evaluate the muscle damage stemming from isometric muscle contraction induced by NMES of low frequency (30 Hz) and high frequency (100 Hz). Methods: Experimental crossover study, randomized, unblinded. The study included 10 male college students, age 24.4 ± 6.0 years, weight 77.1 ± 11.8 kg, height 176.1 ± 5.6 cm, and BMI of 24.8 ± 3 4 kg/m2. Two protocols (A) and (B) with an interval of 7 days between them. (A) - 20 minutes of NMES in the quadriceps at a frequency of 30 Hz (B) - 20 minutes of NMES at a frequency of 100 Hz. Measured lactate, creatine phosphokinase and lactate dehydrogenase before, immediately after, and 6 and 48 hours after the protocols. Results: Comparing 30 Hz vs. 100 Hz the following were observed: lactate (23.7 ± 6.7 vs. 13.4 ± 3.0 mg/dl, p = 0.001); CPK (195.4 ± 116.1 vs. 262.9 ± 153.6 IU, p = 0.022); LDH (374.3 ± 64 vs. 366.6 ± 84.1 IU, ns). The perception of contractile efficiency decreased significantly (p = 0.016) in the 100 Hz Protocol. Conclusion: Both the low-frequency NMES (30 Hz) and the high-frequency (100 Hz) elevate blood markers of muscle damage, most strikingly at the higher frequency. However, the achieved values reflect a normal response to a moderate-intensity exercise.

Muscle damage induced by neuromuscular electrical stimulation (NMES) with frequencies of 30 Hz and 100 Hz