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The challenge of the present work concerned the development and validation of high performance liquid chromatography (HPLC) and UV-Vis spectrophotometric methods for quantitation of methotrexate (MTX) loaded on biodegradable microparticles, composed of copolymers with different solubilities such as chitosan and poly (lactic-co-glycolic acid) (PLGA). The linearity of the analytical curves for MTX were > 0.999 (r2) and the limits of detection (LOD) were 0.014 µg mL-1 and 0.060 µg mL-1 for HPLC and UV-Vis spectrophotometric method, respectively. In addition, both methods were specific, robust, and accurate with a recovery between 89.5% and 105.5%. The method showed precision with relative standard deviations lower than 3.39% for HPLC and 2.90% for UV-Vis spectrophotometry. Statistical analysis revealed that both methods provide equivalent results, and also can be used for quality control of MTX-loaded in drug delivery systems.

HPLC-DAD and UV-Vis Spectrophotometric Methods for Methotrexate Assay in Different Biodegradable Microparticles