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Polystyrene (PS) nanofibers incorporated with ion-pair (IP) reagent, e.g., sodium dodecyl sulfonate (SDSn), were developed as functional adsorbents for the solid-phase extraction (SPE) of ionic neurotransmitter such as 5-hydroxytryptamine (5-HT), prior to the determination by high performance liquid chromatography-fluorescence detection (HPLC-FLD). A comprehensive study was initiated to optimize the preconcentration step by exploring the main factors that affect the extraction/preconcentration efficiency of 5-HT, such as the composition of nanofibers, eluent and its volume, amount of adsorbent, pH and ionic strength. The validity of this method was investigated and optimal analytical performance was achieved including a wide dynamic range of 0.50-200 ng mL-1, detection limits of 0.50 ng mL–1 and precision (as RSD%) lower than 4% for both intra-day and inter-day assays. This method was then applied to the determination of 5-HT in human plasma with satisfactory results.

An Ion-Pair Reagent Incorporated Polystyrene Nanofiber Applied to Solid Phase Extraction of 5-Hydroxytryptamine in Human Plasma