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Partial least squares regression (PLSR) was used for the simultaneous quantification of rifampicin (RIF)and isoniazid (INH) by visible spectrophotometry using a simple derivatization reaction. In the presence of neocuproine, copper(II) is easily reduced by INH to a Cu(I)-neocuproine complex that shows an absorption maximum at 455 nm. Under these conditions, RIF shows an absorption maximum at 449 nm. The calibration set was established between 8and 57 mg L-1 for RIFand 1.5and 7 mg L-1 for INH. The method was applied to the determination of the drugs in urine samples (recoveries between 92and 119%)and in pharmaceutical formulations (relative error lower than 5%).

Simultaneous Determination of Rifampicin and Isoniazid in Urine and Pharmaceutical Formulations by Multivariate Visible Spectrophotometry