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When polybutadiene dissolved in toluene was a binder of carbon powder, the volatility of solvent just after electrode fabrication assured the mechanical solidity of the carbon paste electrode. This characteristic met the qualifications for practical use of carbon paste electrodes. A new enzyme electrode bound with butadiene rubber was constructed. In order to confirm whether it shows quantitative electrochemical behaviors or not, its electrochemical kinetic parameters, e.g. the symmetry factor, the exchange current density, the capacitance of double layer, the time constant, the maximum current, the Michaelis constant and other factors were investigated. These experimental facts showed that butadiene rubber is a recommendable binder for practical use of a carbon paste electrode.

Electrochemical determination of hydrogen peroxide using carbon paste biosensor bound with butadiene rubber