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Currently, many countries have an interest in developing cosmetics materials using native plants. In this aspect, there is increasing need to develop cosmetics materials using native plants in our county. In the present study,calluses were induced from Artemisia annua Linne , which was highlighted because of its useful effects, such as anti-cancer, anti-fungal and anti-inflammation. Water and ethanol extractions were performed from the calluses of Artemisia annua Linne. After the mass production of Artemisia annua Linne' s calluses, water and ethanol extractionwas performed to examine its functional roles in healing wounds and inflammation. The differences in the effective elements were observed in the ethanol extract. The callus showed anti-inflammation activity through the suppressionof the inflammation-related gene, COX-2, and ethanol extracts showed their ability to heal wounds. Overall, these results suggest that the extract of Artemisia annua Linne ’s calluses is a natural and environment-friendly material,and can be used as medical supplies associated with anti-inflammation and healing wounds.

Effect of Artemisia annua Linne callus induced by plant cell culture technology on wound healing