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.            ethylacetate     Sephadex LH-20    Prep-TLC    .  UV spectrum   260 nm   , $^1H$ , $^{13}C$ , HMQC, HMBC NMR ESI mass spectrum     (4'-ethyl-2'-methylfuranyl)-6-methoxy-7-methylnona-2E,4E,6Z,8E-tetraenoic acid     . 【Antifungal and insecticidal activity of Korean traditional medicinal plants was carried out to develop natural material for the development of organic cultural heritage conservation. As a result, Phellodendron amurense fruit was finally selected as a candidate of antifungal and insecticidal natural material. An novel active compound was purified from the ethylacetate fraction of Phellodendron amurense fruits using silica gel and Sephadex LH-20 column chromatography and PTLC. The compound was obtained as yellow oil form; UV ${\lambda}_{max}$ (MeOH): 260 nm. The chemical structure of novel compound was determined as (4'-ethyl-2'-methylfuranyl)-6-methoxy-7-methylnona-2E,4E,6Z,8E-tetraenoic acid on the basis of various NMR experiments including $^1H$ - and $^{13}C$ -NMR, HMQC, HMBC and ESI-mass spectrum.】

A New Compound Isolation and Structure Analysis from Phellodendron Amurense Fruit Extract