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Soybean hull is an attractive feedstock for glucose production. To increase the glucose conversion in acid hydrolysis, a pretreatment method combined steam explosion with alkali pretreatment for soybean hullwas studied. For first step pretreatment, steam explosion conditions (log Ro 2.45) were optimized to obtain maximum solid recovery and cellulose content. In the second step pretreatment, the conditions for potas-sium hydroxide pretreatment of steam exploded soybean hull were optimized by using RSM (response sur-face methodology). The optimum conditions for minimum lignin content were determined to be 0.6% po-tassium hydroxide concentration, 70 °C reaction temperature and 198 min reaction time. The predicted lig-nin content was 2.2% at the optimum conditions. Experimental verification of the optimum conditions gave the lignin content in similar value with the estimated value of the model. Finally, glucose conversion of pretreated soybean hull using acid hydrolysis resulted in 97.1 ± 0.4%. This research of two-step pretreat-ment was a promising method for increasing the glucose conversion in the cellulose-to-glucose process. Keywords : Soybean hull, pretreatment, steam explosion, potassium hydroxide, response surface method-ology

Optimization of Two-stage Pretreatment from Soybean Hull for Efficient Glucose Recovery