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Muscle spindles were investigated using enzyme histochemistry in serial sections of human intrinsic laryngeal muscles obtained from surgical specimens. Muscle spindles were demonstrated in the middle one third of each laryngeal muscle. Five muscle spindles were observed in the interarytenoid, three in the posterior cricoarytenoid, two in the cricothyroid and thyroarytenoid, and one in the lateral cricoarytenoid. The maximum width of the muscle spindles ranged from 35 to 150 rim. Each muscle spindle contained three to eight intrafusal fibers, and the intrafusal fibers ranged from 7.7 to 15.6 pm in diameter. On the basis of enzyme reactivity, intrafusal fibers could be classified into three kinds of muscle fiber, nuclear bags fibers, nuclear bag2 fibers and nuclear chain fibers, which is the same classification observed in other skeletal muscles.

Enzyme-Histochemistry of Muscle Spindles in Human Intrinsic Laryngeal Muscles