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Semen was collected from 10 Black Castellana roosters and the classic sperm variables (ejaculate volume, sperm con - centration and sperm motility) examined. In addition, the hypo-osmotic swelling test was used to investigate sperm cell membrane integrity, and acidic aniline blue staining used to screen for morphological abnormalities (including acrosome integrity) and to examine the condensation status of the chromatin. The latter was also examined by Gram staining. Large andsmallsemenvolumeswereassociatedhighandlowspermconcentrationsrespectively(R 2=0.04,P<0.05).Thepercent - age of motile spermatozoa correlated strongly with the percentage of sperm cells showing an intact acrosome (R 2=0.13, P<0.001) and with the percentage of morphologically normal spermatozoa (R 2=0.04, P<0.05). The percentage of Gram positive spermatozoa was positively correlated with semen appearance (R 2=0.12, P<0.05), sperm cell concentration (R 2=0.13, P<0.05), and with the sperm motility variables studied (R 2=0.14, P<0.05 for percentage mobility, and R 2=0.12,

Sperm variables as predictors of fertility in Black Castellana roosters: use in the selection of sperm donors for genome resource banking purposes