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Avaliou-se a compatibilidade dos herbicidas butafenacil, metribuzim, s-metolacloro, 2,4-D, glifosato, oxassulfurom e trifloxissulfuron no desenvolvimento do fungo entomopatogenico M. anisopliae (isolado IBCB 348), empregado para controle biologico de Mahanarva fimbriolata (Hemiptera: Cercopidae) conhecida como cigarrinhada- raiz da cana. Os herbicidas foram adicionados ao meio de cultura batata dextrose agar (BDA) em temperatura aproximada de 45°C nas doses de campo minimas e maximas recomendadas, fixandose o volume de calda em 200 L/ha. Apos a solidificacao do meio em placas de Petri, o fungo foi inoculado em tres pontos equidistantes. As placas foram incubadas em câmara climatizada avaliandose o diâmetro medio das colonias formadas, o numero de conidios por colonia e a viabilidade dos conidios do fungo apos 20 e 48 horas da inoculacao. Apenas o herbicida oxassulfurom, na menor dose testada (30 g/ha), foi classificado como toxico ao M. anisopliae, sendo os demais classificados em ambas as doses como muito toxicos. Quanto a viabilidade, somente os herbicidas oxassulfurom nas doses de 30 e 60 g/ha nao afetaram a germinacao do fungo ate 20 horas apos a inoculacao e o glifosato a 0,5 e 6,0 L/ha ate 48 horas. Os demais herbicidas mostraram-se incompativeis, afetando o desenvolvimento e a germinacao do M. anisopliae. EFFECT OF HERBICIDES UTILIZED IN SUGAR CANE OF IN VITRO DEVELOPMENT OF ENTOMOPHATOGENIC FUNGI Metarhizium anisopliae (Metsch.) SOROKIN Abstract The compatibility of the herbicides butafenacil, metribuzin, s-metolachlor, 2,4-D, glyphosate, oxasulfuron and trifloxysulfuron in the development of entomopathogenic fungi M. anisopliae (isolate IBCB 348) were evaluated, which is employed in the biological control of Mahanarva fimbriolata (Hemiptera: Cercopidae). The herbicides were added to the culture media potato dextrose agar (PDA) in temperature of approximately 45o C in minimal and maximal recomended field doses, fixing the volume of 200 L/ha. After the solidification of the culture media in Petri dishes, the fungi was inoculated in three equidistant points. The plates were incubated in climatized chamber and the average diameter of the formed colonies were evaluated, the number of conidia per colony and the viability of the conidia after 20 and 48 hours inoculation. Only the herbicide oxasulfuron, in the lowest tested dose (30 g/ha) was classified as toxic to M. anisopliae, being the rest classified in both doses as highly toxic. For viability only the herbicides oxasulfuron in the doses of 30 and 60 g/ha didnt affect the germination of the fungi until 20 hours after inoculation and glyphosate at 0.5 and 6.0 L/ha until 48 hours. The other herbicides were imcompatible, affecting the development and the germination of M. anisopliae.

EFEITO DE HERBICIDAS UTILIZADOS EM CANA-DEAÇÚCAR NO DESENVOLVIMENTO IN VITRO DO FUNGO ENTOMOPATOGÊNICO Metarhizium anisopliae (Metsch.) SOROKIN