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Background: Laparoscopic cholecystectomy is commonly performed for cholelithiasis. In spite of strict aseptic technique and preoperative antibiotic prophylaxis, there is risk of port site wound infection. Aim: To study the incidence of  positive bile culture and the most common pathogen involved  after laparoscopic cholecystectomy in patients of cholelithiasis and its correlation with port site wound infection.   Study design:  Cross sectional study Setting and duration: Bahawal Victoria Hospital Bahawalpur, from 01-01-2019 to 31-12-2020. Method: One hundre patients of cholelithiasis between 18-75 years of age underwent laparoscopic cholecystectomy. Prophylactic antibiotic was given. Bile with gall bladder was collected and sent for culture. Bacterial growth was recorded as positive bile culture. Organism type was recorded. Stone/stones size was also measured.  Data was collected on a proforma which included demographics details like name, age, gender, BMI, clinical features, size of calculi, culture report (positive bile culture if some bacteria is isolated and negative no growth) and type of bacteria isolated and frequency of port site wound infection. Data was analyzed on SPSS version 21.  Results: The mean age of the patients was 42.44±16.58 years and male to female ratio of the patients was 1:1.8. The mean BMI of the patients was 25.93±4.67 kg/m2.  Bile culture for bacteria was positive in 30(30%) patients and the most common organism was E coli 13(43.33%.78%). Positive bile culture was detected in 20 (20%) male patients and 10 female patients. There was significant correlation between positive bile culture and gender (p value &lt; 0.001). Port site wound was infected in 6 (6%) patients and in all the patients bile culture was also positive. Conclusion: Bile culture was positive in 30% patients of cholelithiasis undergoing laparoscopic cholecystectomy and wound infection was present in 6%  patients and all the 6% patients were bile culture positive . Keywords: Cholelithiasis, Culture, Organism, Laparoscopic cholecystectomy

Incidence of Positive Bile Culture and most Common Pathogen involved after Laparoscopic Cholecystectomy in patients of Cholelithiasis