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Sang-HoonLee *,** ,Ki-WonLee*,** ,KyungHeeKim *,Dae-JinYun *,Sang-SooKwak***andByung-HyunLee *ABSTRACTOxidative stress is the main limiting factor in crop productivity. To solve global environmental problemsusing the plant biotechnology, we have developed on the oxidative stress-tolerant transgenic tall fescueplants via Agrobacterium -mediated genetic transformation method. In order to develop transgenic tall fescue(Festucaarundinacea Schreb.) plants with enhanced tolerance to multiple environmental stresses, nucleotidediphosphate kinase gene under the control of CaMV35S promoter were introduced into genome of tallfescue plants. Proteomic analysis revealed that transgenic tall fescue not only accumulated NDP kinase 2protein in their cells, but also induced several other antioxindative enzyme-related proteins. When leaf discsof transgenic plants were subjected to cold stress, they showed approximately 30% less damage thanwild-type plants. In addition, transgenic tall fescue plants showed normal growth when transgenic plantswere subjected to 4 for 3 days treatments. These results suggest that transgene is important in ROSscavenging by induction of antioxidative proteins, and could improve abiotic stress tolerance in transgenictall fescue plants.(Keywords :Tall fescue, Antioxidant gene, Agrobacterium , Transformation)

Characterization of Transgenic Tall Fescue Plants Overexpressing NDP Kinase Gene in Response to Cold Stress