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Marine dinoflagellate Alexandrium minutum producing paralytic shellfish toxins is responsible for paralytic shellfish poisoning (PSP). To investigate its temporal distributions in Chinhae Bay where PSP occurs annually, SYBR Green based Ⅰ A. minutumspecific realtime PCR probe was developed on the LSU rDNA r egion. Assay specificity and sensitivity were tested against related species, and its specificity was further confirmed by sequencing of fieldderived samples. Ten months field survey in 2008 (a total 100 surface water samples) by using the realtime PCR probe showed that A. minutum was detected at very low densities of 14 cells L 1 in May and June being spring in Chinhae Bay, Korea.

Temporal Changes in Abundances of the Toxic Dinoflagellate Alexandrium minutum (Dinophyceae) in Chinhae Bay, Korea