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.            .           .         .                  .           / .  -   /     . -       .        0.010~2.500 mg/mL          .Abstract : In this work, we describe an electrochemical immunosensor for simple, fast andquantitative detection of a urinary hippuric acid (HA). Urinary HA, of molecular weight 180DA, is one of the major metabolites and biological indicators in toluene-exposed humans. Sim-ple and ubiquitous monitoring of exposure to toluene is very important in occupational healthcare. We propose the electrochemical immunoassay based on the dopamine-antigen conjugatefor detecting hippuric acid. Our electrochemical immunoassay system employs a conjugate ofdopamine (DA) as an electrochemical active molecule and hippuric acid (HA) as an antigen.As an electrochemical aspect, dopamine (DA) containing two hydroxyl group can show excel-lent redox signal. Also, dopamine-tethered hippuric acid (DA-HA) shows the reversible redoxsignal in the immunoassay. The competition between HA and DA-HA generated electric signals

Electrochemical Immunoassay based on the Dopamine-antigen Conjugate for Detecting Hippuric Acid