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One of many sources of bromelain is pineapple core. However, in its application as an enzyme-based oral drug, its activity can be reduced due to interactions with gastric fluids. The isolated bromelain from the pineapple core is encapsulated with a hydrogel semi-IPN methyl cellulose-chitosan in order to control its release and to maintain its activity. Isolation of bromelain was conducted with several stages of fractionation with ammonium sulfate salt and dialysis. The bromelain in semi-IPN methyl cellulosechitosan dissolution capability is evaluated in vitro at artificial gastric fluid pH and artificial intestine environment. The specific activity of bromelain obtained from several purification steps shows an increment. The crude enzymes, the ammonium sulfate fraction and the dialysis fractions have specific activity value of 22.39 U/mg, 76.73 U/mg, and 111.72 U/mg, respectively. Hydrogel semi-IPN methyl cellulose-chitosan was used for encapsulation which has the value of crosslinking degree of 46.63% and swelling ratio of 397.59%, respectively. Meanwhile, post loading encapsulation efficiency is 89.47%. The dissolution test results shows proteolytic activity of bromelain can be maintained up to 2.83 U/mL in artificial pH environment. The release rate of bromelain is relatively larger in artificial intestinal environment than artificial gastric fluid.

In Vitro Dissolution Test of Bromelain Isolated from Pineapple Core Encapsulated in Hydrogel Semi-IPN Methyl Cellulose-Chitosan