English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Herein, we present a fully integrated portable centrifugal microsystem for multiplex detection of food poisoning bacteria with a large volume of sample up to 1 mL. The microsystem consists of a portable rotary genetic analyzer and a fully integrated lab-on-a-disc device. The portable rotary genetic analyzer is equipped with a couple of heating blocks, a motor and a UV-Vis optical detector. The device was designed with two units: a 3D printed solution-loading cartridge and a centrifugal microfluidic disc. All the essential solutions for the LAMP reaction (a sample, a washing, an elution and a LAMP cocktail solution) are stored inside the cartridge, and orderly released into centrifugal microdevice by a rotation program. Each unit of the device was designed with 20 reaction chambers for simultaneously detecting 19 kinds of food poisoning bacteria in one test. To increase the amount of a sample to 1 mL, we incorporated the super absorbent polymer (SAP) in the waste chamber to absorb the sample and washing solution during the device operation. The whole process was automatically conducted from bead-based DNA extraction to isothermal DNA amplification by LAMP reaction to colorimetric and UV-vis detection of amplicons in 60 min to identify three kinds of bacteria (Escherichia coli O157:H7, Salmonella Typhimurium, and Vibrio parahaemolyticus).

Identification for a Large-volume Food-borne Bacteria on a Fully Integrated Portable Centrifugal Disc